!Series_title "Astrocytic response to hypoxia" !Series_geo_accession "GSE3045" !Series_status "Public on Mar 16 2006" !Series_submission_date "Aug 02 2005" !Series_last_update_date "Mar 16 2006" !Series_pubmed_id "16507782" !Series_summary "Oxygen is vital for the development and survival of mammals. In response to hypoxia, the brain initiates numerous adaptive responses at the organ level as well as at the molecular and cellular level, including the alteration of gene expression. Astrocytes play critical roles in the proper functioning of the brain, thus the manner in which astrocytes respond to hypoxia is likely important in determining the outcome of brain hypoxia. We used microarray gene expression profiling and data analysis algorithms to identify and analyze hypoxia-responsive genes in primary human astrocytes. We also compared the gene expression patterns in astrocytes with those in human HeLa cells and pulmonary artery endothelial cells (ECs). Remarkably, in astrocytes, 5 times as many genes were up-regulated as down-regulated, whereas in HeLa and pulmonary ECs, as many or more genes were down-regulated as up-regulated. More genes encoding hypoxia-inducible functions, such as glycolytic enzymes and angiogenic growth factors, were strongly induced in astrocytes compared to HeLa cells. The extent of induction was also greater than in HeLa cells. Further, gene ontology and computational algorithms revealed that many target genes of the EGF and insulin signaling pathways and the transcriptional regulators Myc, Jun and p53 were selectively altered by hypoxia in astrocytes and HeLa cells to a varying degree. These results provide a global view of the signaling and regulatory network mediating oxygen regulation in astrocytes, in comparison with that in HeLa cells." !Series_overall_design "Gene expression profiles of three independent batches of astrocytes grown under normoxia were compared to gene expression profiles of three independent batches of astrocytes grown under 1% oxygen for 24 hours. Affymetrix U133 plus 2.0 microarrays were used." !Series_type "response to hypoxia" !Series_contributor "Sarah,,Mense" !Series_contributor "Mei,,Zhou" !Series_contributor "Amit,,Sengupta" !Series_contributor "Changgui,,Lan" !Series_contributor "Galina,,Bentsman" !Series_contributor "David,,Volsky" !Series_contributor "Li,,Zhang" !Series_sample_id "GSM66929 GSM66930 GSM66931 GSM66932 GSM66933 GSM66934 " !Series_contact_name "Sarah,,Mense" !Series_contact_email "smm2123@columbia.edu" !Series_contact_laboratory "Dr. Zhang" !Series_contact_department "Environmental Health Sciences" !Series_contact_institute "Columbia University" !Series_contact_address "722 West 168th Street" !Series_contact_city "New York" !Series_contact_state "NY" !Series_contact_zip/postal_code "10032" !Series_contact_country "USA" !Series_platform_id "GPL570" !Sample_title "astrocytes hypoxia batch 1" "astrocytes hypoxia batch 2" "astrocytes hypoxia batch 3" "astrocytes normoxia batch 1" "astrocytes normoxia batch 2" "astrocytes normoxia batch 3" !Sample_geo_accession "GSM66929" "GSM66930" "GSM66931" "GSM66932" "GSM66933" "GSM66934" !Sample_status "Public on Mar 16 2006" "Public on Mar 16 2006" "Public on Mar 16 2006" "Public on Mar 16 2006" "Public on Mar 16 2006" "Public on Mar 16 2006" !Sample_submission_date "Aug 02 2005" "Aug 02 2005" "Aug 02 2005" "Aug 02 2005" "Aug 02 2005" "Aug 02 2005" !Sample_last_update_date "Mar 16 2006" "Mar 16 2006" "Mar 16 2006" "Mar 16 2006" "Mar 16 2006" "Mar 16 2006" !Sample_type "RNA" "RNA" "RNA" "RNA" "RNA" "RNA" !Sample_channel_count "1" "1" "1" "1" "1" "1" !Sample_source_name_ch1 "astrocyte" "astrocyte" "astrocyte" "astrocyte" "astrocyte" "astrocyte" !Sample_organism_ch1 "Homo sapiens" "Homo sapiens" "Homo sapiens" "Homo sapiens" "Homo sapiens" "Homo sapiens" !Sample_characteristics_ch1 "astrocytes from second trimester human fetal brains" "astrocytes from second trimester human fetal brains" "astrocytes from second trimester human fetal brains" "astrocytes from second trimester human fetal brains" "astrocytes from second trimester human fetal brains" "astrocytes from second trimester human fetal brains" !Sample_molecule_ch1 "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" "total RNA" !Sample_label_ch1 "biotin" "biotin" "biotin" "biotin" "biotin" "biotin" !Sample_hyb_protocol "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" !Sample_scan_protocol "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" "standard Affymetrix procedures" !Sample_description "astrocytes from second trimester (14-19 weeks) human fetal brains" "astrocytes from second trimester (14-19 weeks) human fetal brains" "astrocytes from second trimester (14-19 weeks) human fetal brains" "astrocytes from second trimester (14-19 weeks) human fetal brains" "astrocytes from second trimester (14-19 weeks) human fetal brains" "astrocytes from second trimester (14-19 weeks) human fetal brains" !Sample_data_processing "Affymetrix Microarray Suite version 5.0" "Affymetrix Microarray Suite version 5.0" "Affymetrix Microarray Suite version 5.0" "Affymetrix Microarray Suite version 5.0" "Affymetrix Microarray Suite version 5.0" "Affymetrix Microarray Suite version 5.0" !Sample_platform_id "GPL570" "GPL570" "GPL570" "GPL570" "GPL570" "GPL570" !Sample_contact_name "Sarah,,Mense" "Sarah,,Mense" "Sarah,,Mense" "Sarah,,Mense" "Sarah,,Mense" "Sarah,,Mense" !Sample_contact_email "smm2123@columbia.edu" "smm2123@columbia.edu" "smm2123@columbia.edu" "smm2123@columbia.edu" "smm2123@columbia.edu" "smm2123@columbia.edu" !Sample_contact_laboratory "Dr. Zhang" "Dr. Zhang" "Dr. Zhang" "Dr. Zhang" "Dr. Zhang" "Dr. Zhang" !Sample_contact_department "Environmental Health Sciences" "Environmental Health Sciences" "Environmental Health Sciences" "Environmental Health Sciences" "Environmental Health Sciences" "Environmental Health Sciences" !Sample_contact_institute "Columbia University" "Columbia University" "Columbia University" "Columbia University" "Columbia University" "Columbia University" !Sample_contact_address "722 West 168th Street" "722 West 168th Street" "722 West 168th Street" "722 West 168th Street" "722 West 168th Street" "722 West 168th Street" !Sample_contact_city "New York" "New York" "New York" "New York" "New York" "New York" !Sample_contact_state "NY" "NY" "NY" "NY" "NY" "NY" !Sample_contact_zip/postal_code "10032" "10032" "10032" "10032" "10032" "10032" !Sample_contact_country "USA" "USA" "USA" "USA" "USA" "USA" !Sample_supplementary_file "NONE" "NONE" "NONE" "NONE" "NONE" "NONE" !Sample_data_row_count "54675" "54675" "54675" "54675" "54675" "54675" !series_matrix_table_begin "ID_REF" "GSM66929" "GSM66930" "GSM66931" "GSM66932" "GSM66933" "GSM66934" "1007_s_at" 7889.6 7786.3 7841.1 4556.7 7353.4 8767.5 "1053_at" 161.7 98.5 187.9 212.7 230.4 116.4 "117_at" 56 49 20.7 60.5 87.5 116.3 "121_at" 540.9 269.8 687.8 669.4 835.3 943.6 "1255_g_at" 81.1 16.5 37.2 113.3 45.3 29.9 "1294_at" 81 66.1 104.2 168.3 104.2 79.6 "1316_at" 220.1 89.7 58.4 78.5 156 130 "1320_at" 15.6 75.2 102.7 90.2 25 27 "1405_i_at" 9.5 29.6 7.5 4.2 8 7.3 "1431_at" 29.8 66.2 76.3 51.8 107.9 49 "1438_at" 385.4 122.2 118.4 537.8 138.2 171.7 "1487_at" 197 174.9 223.8 248.4 246.4 246.7 "1494_f_at" 86.6 73 67.5 116.7 156.2 148.6 "1552256_a_at" 464.3 483 386 395.8 461 338.4 "1552257_a_at" 579.4 421.1 522.2 522.5 513.1 504.1 "1552258_at" 211.9 80 80.7 185.7 167.4 105.4 "1552261_at" 12.8 50.6 20.4 45.3 9.4 54.6 "1552263_at" 207.9 86.6 89.9 179.5 77.5 71.8 "1552264_a_at" 474.1 327.5 716.5 532.8 255.4 370.2 "1552266_at" 84.7 69.7 80.5 71.7 105.9 74.4 "1552269_at" 6.1 39.9 5.5 10.1 8.9 9.3 "1552271_at" 23.2 23.7 16.3 11.7 28.2 17.2 "1552272_a_at" 55.3 62 28 70.3 97.4 118.4 "1552274_at" 402.2 321.7 223.3 269.6 323.3 208.5 "1552275_s_at" 284.4 665.6 255.9 203.6 259.3 131.3 "1552276_a_at" 85.4 14.8 124.8 70.2 50.7 13.4 "1552277_a_at" 1176.5 990.7 642.9 573 715 736.3 "1552278_a_at" 75.5 3.9 9.3 53.3 76 109.5 "1552279_a_at" 168.4 96 186.4 195.7 264.6 150.6 "1552280_at" 26.6 7.6 6.9 8.6 27 12 "1552281_at" 62.1 102.1 60.1 102.6 172.5 136.5 "1552283_s_at" 235.5 145.2 106.6 189.6 108.1 81.2 "1552286_at" 14.3 120.9 135.1 50.2 111.4 172.6 "1552287_s_at" 206.3 515.9 427.3 331.2 661.8 406.9 "1552288_at" 32.2 13.5 14.7 13 23 90.8 "1552289_a_at" 106.3 34.6 5.9 63.9 69.9 72.7 "1552291_at" 126.5 127.4 295.8 141.7 165.3 251.6 "1552293_at" 11.2 23.8 6.5 8 35.6 29.8 "1552295_a_at" 736.8 564.8 718 432.3 902 1219.7 "1552296_at" 19.1 34.1 30.2 10.7 8.6 48.5 "1552299_at" 65 102.6 79.5 119.9 100.6 60.3 "1552301_a_at" 154.5 94.5 61.6 137.2 109.3 140.7 "1552302_at" 52.5 10.5 26.9 31.3 3.2 41.7 "1552303_a_at" 68.4 62 38 10.8 31.5 18.2 "1552304_at" 46.4 46.3 29.8 48.6 62.9 37.8 "1552306_at" 24.7 24 20.2 74.6 16.3 81.5 "1552307_a_at" 304.1 92.9 209.9 141 132 143.5 "1552309_a_at" 160.2 522.5 1205.1 249.1 755.5 1155.3 "1552310_at" 195.9 278.8 236 285.4 253.3 187.6 "1552311_a_at" 85.7 62.8 71.2 13.4 32.7 173.8 "1552312_a_at" 165.8 137.1 389.1 285.5 189.3 396.9 "1552314_a_at" 93.3 35.4 22 53 70.4 115.9 "1552315_at" 83.3 83.7 4.6 4.8 30.2 58.4 "1552316_a_at" 28.6 2.8 14.9 1.8 39 3.4 "1552318_at" 61 19.4 8.7 5.5 51.7 13 "1552319_a_at" 91.8 39.8 8.5 21.4 69 194.2 "1552320_a_at" 9.6 35.4 81.9 17.7 63.4 69.1 "1552321_a_at" 6.2 23.1 16.6 7.6 1.7 7 "1552322_at" 16 41.6 20.5 14.2 3.2 10 "1552323_s_at" 59.7 93 98.8 102.2 132.8 132.1 "1552325_at" 34.8 16.5 1.2 13.5 19 63.8 "1552326_a_at" 76.6 11.5 86.9 51.2 8.3 105.5 "1552327_at" 95 234.8 193.4 116.6 184.5 58.7 "1552329_at" 237.6 219.9 329.5 282.1 276.6 208 "1552330_at" 86.5 111.4 123 59.5 213.8 73.2 "1552332_at" 152.3 60.5 40.5 40 30 211.6 "1552334_at" 369.5 278.8 259 143.4 211.4 320 "1552335_at" 14.6 38.3 48.7 45.6 10.8 13.3 "1552337_s_at" 11.9 4.5 78.4 26.1 4.7 11.2 "1552338_at" 3.2 2.4 1.7 3.4 2.9 4.5 "1552340_at" 32.4 3.5 3.2 3.2 50 17.5 "1552343_s_at" 141.2 70.4 66.3 38.5 130.3 166.6 "1552344_s_at" 213.1 262.6 262 365.9 171.4 174.8 "1552347_at" 1298.1 1294.5 948 441.3 673.5 671.5 "1552348_at" 310.8 166.4 290.6 392.7 211.7 136 "1552349_a_at" 131.1 65 72.5 67.9 57 140.1 "1552354_at" 11.6 13.5 9.8 2.2 18.8 42.7 "1552355_s_at" 7.8 2.4 7.4 8.2 19.1 18.2 "1552359_at" 5.3 0.6 18.4 32 20 39.3 "1552360_a_at" 74.5 53.3 70.4 74 99.2 89.5 "1552362_a_at" 205.2 85.1 91.8 147.3 189.4 134.1 "1552364_s_at" 1149.7 397.1 376.9 992.1 139.3 198.8 "1552365_at" 5.1 20.6 33.2 27.3 1.5 26.7 "1552367_a_at" 10.2 1.4 1.9 1.4 2.1 6.2 "1552368_at" 42 40 7.1 3.6 12.3 117.3 "1552370_at" 73.3 128.4 94.2 138.9 98.8 133.9 "1552372_at" 20.5 16.6 13.8 19 6.9 17.2 "1552373_s_at" 12.9 31.8 23.4 12.1 37.1 26 "1552375_at" 71.1 102.1 113.6 135.8 115.2 144.6 "1552377_s_at" 252.6 133.7 195.1 249.6 269 218.4 "1552378_s_at" 237.4 373.1 210.4 30.8 42.4 193.2 "1552379_at" 14.5 3.4 2.4 0.7 7.2 48.7 "1552381_at" 46.8 62.6 87.6 98.9 63.7 66.5 "1552383_at" 118.8 107.4 111.4 70.3 59.4 251.6 "1552384_a_at" 138.4 13.9 41.4 7.1 8.6 100.3 "1552386_at" 32.6 33.5 17.8 34.4 33.4 21.9 "1552388_at" 19.3 16.1 22.5 17.9 16.3 31.2 "1552389_at" 19.2 1.3 10.6 10.2 35.4 24.1 "1552390_a_at" 6.8 1.5 31.1 22.5 17.3 27.8 "1552391_at" 15.4 1.5 29.7 17.4 7.4 61.2 "1552393_at" 5.2 21.4 20.7 3.3 21.3 43 "1552394_a_at" 3.4 2.4 3.6 7 7.6 26.5 "1552395_at" 28.8 8.5 13 19.3 31.3 37.4 "1552396_at" 82.2 6 8.2 12 12.5 61.4 "1552398_a_at" 4.8 2.9 6.6 5.1 37.4 7.5 "1552399_a_at" 49 12.2 21.9 80.3 50.3 35.3 "1552400_a_at" 85 58.6 39.8 55.3 73.9 105.9 "1552401_a_at" 42.2 21.7 47.9 16.3 56.5 93.8 "1552402_at" 7.2 3.2 43.9 4.6 14.4 52.3 "1552405_at" 38.6 25.8 12.2 13.6 17 66.3 "1552408_at" 50.7 23.2 57.2 29.3 16.6 51 "1552409_a_at" 12.1 62.1 12.8 10.3 70 145.8 "1552410_at" 104.5 40.7 72.2 76.5 102.6 102.8 "1552411_at" 426.7 133.9 245.1 293.4 540 514.9 "1552412_a_at" 5.9 24.7 3.1 9.5 8.4 13 "1552414_at" 52.9 50 20.8 4.8 52.7 159.2 "1552415_a_at" 17.5 73 38.8 13.9 45.4 100.8 "1552417_a_at" 257 139.4 365.1 527.3 243.5 227 "1552418_at" 15.4 5.5 5.2 3.2 12.5 21.5 "1552419_s_at" 181.1 164.9 164.1 154.7 348.3 295.5 "1552421_a_at" 9.9 3.5 5.8 2.8 9.3 9.5 "1552422_at" 30.2 53.8 17.7 11 49.5 34.2 "1552423_at" 207.4 251.9 106.2 125.3 158.6 246 "1552424_at" 5.4 5.1 2 11.9 2 3.4 "1552425_a_at" 75.3 43.1 75.7 18.6 95.9 93.5 "1552426_a_at" 934 778.6 1572.5 704 590.7 1061.5 "1552427_at" 38.9 17.6 36.9 22.5 19.6 29.1 "1552430_at" 159.6 83.1 56.1 85.4 77.3 37.5 "1552432_at" 5.2 4.6 7 6.9 10.2 7.3 "1552436_a_at" 3.9 7.6 21 9.6 2.8 6.8 "1552438_a_at" 45.2 52.2 32.2 47.3 91.8 151 "1552439_s_at" 174.6 1301.9 1428.7 271.1 1578.2 1103.3 "1552440_at" 30.7 47.6 30.2 10.7 51.6 91.6 "1552445_a_at" 77.8 13.1 9.5 5.6 8.5 5.8 "1552448_a_at" 4.4 13.3 17.8 2.2 23.8 10.6 "1552449_a_at" 7.1 1.9 4.4 3.5 1.8 4.4 "1552450_a_at" 66 55.2 58.6 66.5 94 34.6 "1552452_at" 6.1 26.4 4 1.3 6.9 2.9 "1552453_a_at" 5.5 2.4 4 2.2 3 4.8 "1552455_at" 303.1 310.5 227.9 86.7 323.2 196.7 "1552456_a_at" 5.1 28.2 3.3 5.4 17.6 19.2 "1552457_a_at" 10.7 12.1 28.8 32.4 9.4 20.1 "1552458_at" 23.1 34.6 16.9 28.8 12.6 6 "1552459_a_at" 12.4 20.6 13.8 6.5 26.7 16.6 "1552461_at" 35.5 22.8 16.6 22.2 22.2 46.8 "1552463_at" 46.9 32.5 60.2 34.4 74.3 104.4 "1552466_x_at" 25.5 1.4 35.5 12.6 3.3 3.9 "1552467_at" 97.3 80.2 19.6 19.5 37.4 134.2 "1552468_a_at" 25.2 16.6 24.4 52.5 76.3 115.8 "1552470_a_at" 26.2 46.6 38.6 12.8 7.3 49 "1552472_a_at" 338 235.6 212.4 237.8 275.4 242 "1552473_at" 27.5 71.7 30.4 37.1 25.2 14.3 "1552474_a_at" 379.5 378.8 287.5 394.9 279.8 398.8 "1552476_s_at" 952.4 531.6 459 234.2 815.9 463.2 "1552477_a_at" 52.7 8.6 20.5 36.9 8.1 35.3 "1552478_a_at" 5.1 2 17.8 12.8 53.5 12.2 "1552480_s_at" 4.2 12.5 8.7 19.1 5.9 19.3 "1552481_s_at" 86.3 70.7 193.6 75.4 144.3 215.3 "1552482_at" 221.3 146 187.8 144.6 187 237.1 "1552484_at" 20.7 8.4 15.9 70.5 33 40.4 "1552485_at" 408.8 386.6 304.4 204.3 211.7 423.9 "1552486_s_at" 148.2 97.8 130.2 68.7 53.3 76.7 "1552487_a_at" 15 26.5 10.3 22.8 34.6 2 "1552489_s_at" 4.7 8.8 20.1 2.6 34.5 22.2 "1552490_at" 46.1 40.7 86 46 119.8 64.1 "1552491_at" 14.7 33.4 6.9 11.4 154.3 14.3 "1552493_s_at" 3.2 4.8 4.9 6.7 6.3 18.3 "1552494_at" 27.6 9 14.6 16.2 12.2 3.2 "1552496_a_at" 53.7 105 189.7 6.5 17.2 182.9 "1552497_a_at" 169.8 79.5 161.6 130.1 443.4 221.9 "1552498_at" 21.2 34.7 55.2 44.7 59 56.8 "1552499_a_at" 56.1 43.7 58.4 74.2 61.2 83.2 "1552501_a_at" 10.3 26.6 8.9 73.3 38.4 23.2 "1552502_s_at" 38.3 25.4 67.6 33.9 37.6 50.9 "1552503_at" 11.7 34.9 10.8 17.3 66.7 46 "1552504_a_at" 78.6 99.5 112.9 118.2 126.1 114.3 "1552506_at" 240.7 434.6 639.2 211.7 469.3 736.8 "1552507_at" 294.5 112.7 185.8 78.8 179.7 119.5 "1552508_at" 165.8 52.6 70.7 49 71.8 37.6 "1552509_a_at" 33.3 58 11.7 34.1 129.1 64.6 "1552510_at" 461.2 247.9 210.4 434 520.4 496.4 "1552511_a_at" 47.8 44.5 74.9 57.1 5.6 8.8 "1552514_at" 73.9 98.3 37.3 34.6 45.2 42.2 "1552515_at" 66.8 34.7 32.1 35.2 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